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MigraIso™︎ - Human Blood Neutrophil-Derived Migrasome Extraction Kit
PRHB002S
For enriching and purifying neutrophil-derived migrasomes from human peripheral blood or plasma, with one test defined as a crude preparation totaling OD280=100.
Product manualUse this page for product selection and experiment planning. Consult the PRHB002 series manual for the full procedure and limitations.
Components and storage
| Component | Qty. | Storage |
|---|---|---|
| Optimization reagent A | PRHB002: 20 μLPRHB002S: 5 μL | Store at -20°C. Promoting reagent C is a suspension; visible sediment is normal and must be mixed thoroughly before use. |
| Enhancing reagent B | PRHB002: 112 μLPRHB002S: 28 μL | Store at -20°C. Promoting reagent C is a suspension; visible sediment is normal and must be mixed thoroughly before use. |
| Promoting reagent C | PRHB002: 32 μLPRHB002S: 8 μL | Store at -20°C. Promoting reagent C is a suspension; visible sediment is normal and must be mixed thoroughly before use. |
| Buffer D | PRHB002: 25 mLPRHB002S: 5 mL | Store at 4°C. Do not freeze or allow the reagents to ice. |
| Diluent E | PRHB002: 80 mLPRHB002S: 20 mL | Store at 4°C. Do not freeze or allow the reagents to ice. |
| Purification reagent F | PRHB002: 120 μLPRHB002S: 30 μL | Store at 4°C. Do not freeze or allow the reagents to ice. |
| Purification reagent G | PRHB002: 20 μLPRHB002S: 5 μL | Store at 4°C. Do not freeze or allow the reagents to ice. |
| Purification reagent H | PRHB002: 80 μLPRHB002S: 20 μL | Store at 4°C. Do not freeze or allow the reagents to ice. |
| Magnetic bead suspension I | PRHB002: 600 μLPRHB002S: 150 μL | Store at 4°C. Do not freeze or allow the reagents to ice. |
| Magnetic bead suspension J | PRHB002: 100 μLPRHB002S: 25 μL | Store at 4°C. Do not freeze or allow the reagents to ice. |
| 15 mL centrifuge tubes | PRHB002: 20 tubesPRHB002S: 5 tubes | Store as directed in the product manual. |
| Instruction manual | PRHB002: 1 copyPRHB002S: 1 copy | Store as directed in the product manual. |
PRODUCT FEATURES
Product advantages
Neutrophil-specific purification
Multistep enrichment and immunomagnetic negative selection recover human neutrophil-derived migrasomes.
Low contamination, consistent quality
Purification reduces non-target components and protein contamination to obtain migrasome samples of consistent, reliable quality.
Broad sample compatibility
Suitable for human peripheral blood and fresh plasma; plasma stored at -80°C can also be processed as directed in the manual.
Broad downstream use
Supports migrasome counting, protein and RNA analysis, and cell-function studies.
SAMPLE REQUIREMENTS
Sample requirements
Fresh plasma may be used. When starting from peripheral blood, sodium citrate collection tubes are recommended. Peripheral blood intended for plasma preparation must not be stored for more than 4 hours before migrasome extraction.
Plasma stored at -80°C may be used, but freeze-thawing and plasma-preparation conditions may affect output and yield. Plasma obtained through blood-filtration membranes is not suitable.
EDTA-anticoagulated samples may be processed, but platelet contamination may increase. A fixed-angle rotor of 45° or greater is recommended for 20,000×g enrichment.
EXPERIMENTAL WORKFLOW
Workflow
EXAMPLE RESULTS
Example results
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FAQ
Frequently asked questions
Which samples are suitable?
Human peripheral blood or plasma is suitable, and fresh plasma is recommended. Samples stored at -80°C may be used, but freeze-thawing and plasma-preparation conditions may affect output and yield. Plasma obtained through blood-filtration membranes is not suitable.
Which anticoagulant is recommended?
Use sodium citrate collection tubes. EDTA-anticoagulated blood has been tested but showed more platelet contamination. Other anticoagulants have not been tested; evaluating their suitability before use is recommended.
How long may fresh peripheral blood be held?
Peripheral blood used to prepare plasma should not be held for more than 4 hours before migrasome extraction because hemolysis may substantially affect separation.
How is one test defined?
One test is a crude preparation totaling OD280=100, calculated as A280 multiplied by sample volume in μL. PRHB002 and PRHB002S provide 20 and 5 tests, respectively.
How should centrifuge rotors be selected?
Use a swinging-bucket rotor when practical for low-speed plasma preparation and a fixed-angle rotor of 45° or greater for 20,000×g enrichment.
Manual and COA
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