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MigraIso™︎ Mouse Total Blood Cell-Derived Migrasome Isolation Kit
PRMB001
For enriching and purifying total cell-derived migrasomes from mouse blood or plasma, with current packs covering 20 mL or 5 mL of plasma.
Product manualUse this page for product selection and experiment planning. Consult the PRMB001 series manual for the full procedure and limitations.
Components and storage
| Component | Qty. | Storage |
|---|---|---|
| Buffer A | PRMB001: 25 mLPRMB001S: 5 mL | Store as directed in the product manual. |
| Diluent B | PRMB001: 80 mLPRMB001S: 20 mL | Store as directed in the product manual. |
| Purification reagent C | PRMB001: 160 μLPRMB001S: 40 μL | Store as directed in the product manual. |
| Magnetic bead suspension D | PRMB001: 400 μLPRMB001S: 100 μL | Store as directed in the product manual. |
| 15 mL centrifuge tubes | PRMB001: 20 tubesPRMB001S: 5 tubes | Store as directed in the product manual. |
| Instruction manual | PRMB001: 1 copyPRMB001S: 1 copy | Store as directed in the product manual. |
PRODUCT FEATURES
Product advantages
Total cell-derived migrasome isolation
Suitable for isolating total cell-derived migrasomes from mouse blood or plasma.
Low contamination, consistent quality
Multistep enrichment, purification, and magnetic-bead negative selection reduce non-target components and protein contamination.
Flexible sample types
Suitable for mouse whole blood, plasma, or serum; samples stored at -80°C can also be processed as directed in the manual.
Broad downstream applications
Suitable for migrasome counting and imaging, protein and RNA analysis, and cell function studies.
SAMPLE REQUIREMENTS
Sample requirements
Samples stored at -80°C may be used, but freeze-thawing and plasma-preparation conditions may affect yield and recovery. Plasma obtained through blood-filtration membranes is not suitable.
Low-binding consumables are recommended throughout the procedure. A fixed-angle rotor of 45° or greater is recommended for 20,000×g enrichment.
EXPERIMENTAL WORKFLOW
Workflow
FAQ
Frequently asked questions
Which samples are suitable?
Mouse peripheral blood, plasma, or serum is suitable, including plasma or serum stored at -80°C. Freeze-thawing and plasma-preparation conditions may affect yield and recovery. Plasma obtained through blood-filtration membranes is not suitable.
Which anticoagulant is recommended?
The manual recommends EDTA collection tubes. Sodium-citrate blood has been tested but yielded less than EDTA; other anticoagulants require user evaluation.
How long may fresh peripheral blood be held?
Peripheral blood should not be held for more than 4 hours before migrasome extraction because hemolysis may substantially affect separation.
How can platelet contamination be reduced?
Minimize mechanical disturbance of blood, conduct low-speed plasma preparation at room temperature, and keep enrichment and purification stages at 4°C. If contamination is severe, repeat the purification workflow as directed.
Manual and COA
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