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MigraIso™︎ Mouse Blood Neutrophil-Derived Migrasome Isolation Kit
PRMB002
For enriching and purifying neutrophil-derived migrasomes from mouse blood or plasma, with current packs covering 20 mL or 5 mL of plasma.
Product manualUse this page for product selection and experiment planning. Consult the PRMB002 series manual for the full procedure and limitations.
Components and storage
| Component | Qty. | Storage |
|---|---|---|
| Buffer A | PRMB002: 25 mLPRMB002S: 5 mL | Store as directed in the product manual. |
| Diluent B | PRMB002: 80 mLPRMB002S: 20 mL | Store as directed in the product manual. |
| Purification reagent C | PRMB002: 160 μLPRMB002S: 40 μL | Store as directed in the product manual. |
| Purification reagent D | PRMB002: 80 μLPRMB002S: 20 μL | Store as directed in the product manual. |
| Purification reagent E | PRMB002: 160 μLPRMB002S: 40 μL | Store as directed in the product manual. |
| Magnetic bead suspension F | PRMB002: 400 μLPRMB002S: 100 μL | Store as directed in the product manual. |
| Magnetic bead suspension G | PRMB002: 320 μLPRMB002S: 80 μL | Store as directed in the product manual. |
| 15 mL centrifuge tubes | PRMB002: 20 tubesPRMB002S: 5 tubes | Store as directed in the product manual. |
| Instruction manual | PRMB002: 1 copyPRMB002S: 1 copy | Store as directed in the product manual. |
PRODUCT FEATURES
Product advantages
Neutrophil-specific purification
Isolation and purification of mouse neutrophil-derived migrasomes support studies of their cellular origin.
Low contamination, consistent quality
Multistep purification and dual magnetic-bead negative selection reduce non-target components and protein contamination to support sample quality.
Flexible sample types
Suitable for mouse whole blood, plasma, or serum; samples stored at -80°C can also be processed as directed in the manual.
Broad downstream applications
Isolated samples can be used for migrasome counting, protein and RNA analysis, and cell function assays.
SAMPLE REQUIREMENTS
Sample requirements
Samples stored at -80°C may be used, but freeze-thawing and plasma-preparation conditions may affect yield and recovery. Plasma obtained through blood-filtration membranes is not suitable.
Low-binding consumables are recommended throughout the procedure. A fixed-angle rotor of 45° or greater is recommended for 20,000×g enrichment.
EXPERIMENTAL WORKFLOW
Workflow
EXAMPLE RESULTS
Example results
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FAQ
Frequently asked questions
How does this product differ from PRMB001?
PRMB002 targets mouse neutrophil-derived migrasomes and uses an additional purification stage plus a two-bead system, whereas PRMB001 targets total cell-derived migrasomes.
Which samples are suitable?
Mouse peripheral blood, plasma, or serum is suitable, including plasma or serum stored at -80°C. Freeze-thawing and plasma-preparation conditions may affect yield and recovery. Plasma obtained through blood-filtration membranes is not suitable.
Which anticoagulant is recommended?
The manual recommends EDTA collection tubes. Sodium-citrate blood has been tested but yielded less; other anticoagulants require user evaluation.
How long may fresh peripheral blood be held?
Peripheral blood should not be held for more than 4 hours before migrasome extraction because hemolysis may substantially affect separation.
Manual and COA
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